Thermodynamics of nucleic acids


Temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). The melting temperature is defined as the temperature at which half of the DNA strands are in the random coil or single-stranded (ssDNA) state. Tm depends on the length of the DNA molecule and its specific nucleotide sequence. DNA, when in a state where its two strands are dissociated.



 


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