Nucleic Acid Probes

In addition to detecting mRNA, nucleic acid probes are also employed to evaluate the interphase chromosomal status of tumour cells. Under circumstances that permit the probe sequence to hybridise with its complementary sequence, the probe is brought into contact with the sample. Probes range in length from small oligonucleotides to mutagenic chromosomal regions cloned into bacteria, and they can be made of either DNA or RNA.

The use of nucleic acid probes is based on the identification of distinctive nucleotide sequences in a microorganism's DNA or RNA; these distinctive nucleotide'signatures' serve as substitutes for the presence of the organism itself. Bacterial ribosomes are crucial organelles that play a key role in the process of protein synthesis. They are highly conserved

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