A New Approach to Determining the Nucleic Acid Sequence


Innovative sequencing process that should be fully automated. A nucleotide or deoxynucleotide sequence is broken up into short pieces in this process, and the fragments are then quantitated by hybridising to oligodeoxynucleotides on a solid platform.



uses oligonucleotide hybridization techniques to extract local sequence information from a DNA fragment, and then uses the resulting information to reconstruct the sequence. The most likely DNA fragment had to produce the set of hybridization probes, according to the rates of false positive and false negative hybridization, and then we may estimate the likelihood that this DNA fragment produced the hybridization data.



Fragmentation



Sequences



Hybridization



Reconstructs



 


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